Catch - Attack

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 We hypothesized that PSIIRC should be a major target

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fibre7orange



Messages : 612
Date d'inscription : 22/01/2013

MessageSujet: We hypothesized that PSIIRC should be a major target   Lun 5 Fév - 6:26

The goal of our research is to genetically engineer novel, mesophilic cyanobacteria that retain prolonged photosynthetic activity and biomass production under continuous illumination at elevated temperatures to which the Y-27632 ROCK inhibitor ‘‘wild type’’ cannot adjust. We hypothesized that PSIIRC should be a major target for such engineering and that genetic differences between the PSIIRC in thermophiles and mesophiles provide clues for new strategies. Following sequence alignment analysis, we found two sites within a motif in the D1 protein subunit that are consistently occupied by different residues in thermophiles and mesophiles. In a previous study, we focused on the effect of single mutation on the electron transfer dynamic with respect to the ambient BAY 73-4506 VEGFR/PDGFR inhibitor temperature. Here, we aimed at deciphering the mutations effect on the bacterium viability at above its physiological temperature. Therefore, a double mutation was performed in like motif, which made it identical to the one found in the thermophilic cyanobacterium T. elongatus. Indeed, only a slight decrease in the growth rate was shown by the temperatures at which the DKS grew at a much slower rate.
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